The use of Spheroids in the Study of Invasion
Rolf Bjerkvig in Spheroid Culture in Cancer Research, 2017
Our interest in the MCF-7 human breast carcinoma cell line stems from the availability of both invasive and noninvasive variants. MCF-7/AZ (obtained from Dr. P. Briand, Fibiger Institute, Copenhagen, Denmark) and MCF/LP (obtained from Dr. L. Plessers, Limburg University Center, Diepenbeek, Belgium) are examples of noninvasive MCF-7 cell variants. Examples of invasive variants are MCF-7/6 (obtained from Dr. H. Rochefort, Unité d’Endocrinologie Cellulaire et Moléculaire, Montpellier, France) and MCF-7/JA (Janssen Pharmaceutica, Department of Life Sciences, Beerse, Belgium). The difference between invasive and noninvasive variants is clearly observed after 8 d of confronting culture (Figure 5). We have chosen MCF-7/AZ and MCF-7/6 to investigate for invasion-related differences. Both variants were extensively characterized via their lactate dehydrogenase pattern, their expression of hormone receptors, their pattern of intermediate filaments, and their immunoreactivity with monoclonal antibodies that specifically recognize MCF-7 plasma membrane antigens, and can be used to differentiate MCF-7 from other human breast cells.79
The Gene for t-PA
Cornelis Kluft in Tissue-Type Plasminogen Activator (t-PA): Physiological and Clinical Aspects, 1988
Steroid hormones are small, hydrophobic molecules derived from cholesterol that can bind reversibly to specific hormone-receptor proteins in the cytoplasm.102 The human cell line MCF-7, derived from a metastatic breast cancer, responds to estrogen. It contains cytoplasmic estrogen receptors that, in the presence of the hormone, are capable of translocating to the nucleus and inducing protein synthesis. MCF-7 cells produce both t-PA and u-PA. Treatment with estradiol or testosterone induces the production of t-PA, but has no effect on u-PA. The induction of t-PA is blocked by an estrogen antagonist, indicating that stereospecific receptors are involved. The induction requires both RNA and protein synthesis, indicating that the effect may be on the gene level.103
Breast Imaging with 99mTc-Methylenediphosphonate
Raymond Taillefer, Iraj Khalkhali, Alan D. Waxman, Hans J. Biersack in Radionuclide Imaging of the Breast, 2021
The evaluation of the 99mTc-MDP uptake in human breast cancer cell lines is under investigation in our laboratory. 99mTc-MDP uptake was evaluated at the following time points: 1,5, 10, 40, 60, and 120 min either at 4 and 37°C. 99mTc pertechnetate was used as control. For these experiments human breast cancer cell line MCF-7 was used. The cells were detached from the flasks and initially resuspended in 0.5 mL of RPM1 1640 medium (500,000 cells/tube), centrifuged, and washed twice with phosphate-buffered saline (PBS). The pellets containing MCF-7 cells were then resuspended in 0.5 mL of PBS and incubated with 10 μCi in 10 (iL of 99mTc-pertechnetate and 99mTc-MDP at 4 and 37°C. After incubation the tubes were centrifuged and both pellet and supernatant were counted in a y counter. Duplicates were obtained for each experimental time point. 99mTc-MDP was concentrated in negligible amounts within human breast cancer cell lines MCF-7. Our preliminary data mirror the results of a previous study, recently published (24). However, the 99mTc-MDP cell-related activity was initially (1-10 min) higher (five- to seven-fold) than the 99mmTc-pertechnetate activity, suggesting that a very early, not stable binding to the cell membranes occurs. In fact, in none of our experiments, as well as in other studies, is 99mTc-MDP internalized within the cells as occurs for sestamibi (24).
Isolation and identification of three new chromones from the leaves of Pimenta dioica with cytotoxic, oestrogenic and anti-oestrogenic effects
Published in Pharmaceutical Biology, 2018
Brian J. Doyle, Temitope O. Lawal, Tracie D. Locklear, Lorraina Hernandez, Alice L. Perez, Udeshi Patel, Shitalben Patel, Gail B. Mahady
Human gastric cancer cells, AGS and NCI-N87 were purchased from ATCC (Manassas, VA). Human breast adenocarcinoma cells, MCF-7 were a kind gift from Dr. Hyun-Young Jeong of the Department of Pharmacy Practice, UIC. It was grown and maintained in minimum essential medium Eagle with Earle’s salt and l-glutamine (MEM 1X; Corning Cellgrow, Manassas, VA). AGS (CRL-1739) was obtained from ATCC, grown and maintained in Kaighn’s modification of Ham’s F-12 with l-glutamine (ATCC). NCI-N87 obtained from ATCC was grown and maintained in RPMI 1640 medium (Gibco, Life Technology, Grand Island, NY). All growth media were supplemented with 10% FBS (Gibco, Life Technology, Grand Island, NY) and 1% penicillin/streptomycin (Gibco, Life Technology, Grand Island, NY). The cells were incubated at 37 °C in a humidified atmosphere of 5% CO2 and 95% air. At 80% confluency, the cells were harvested by adding 0.25% trypsin/EDTA and counted by means of trypan blue and haemocytometer. These cells were then re-suspended at appropriate concentration and plated for cellular assays.
Anticancer activity of linalool: comparative investigation of ultrastructural changes and apoptosis in breast cancer cells
Published in Ultrastructural Pathology, 2022
Hulya Elbe, Feral Ozturk, Gurkan Yigitturk, Tuba Baygar, Turker Cavusoglu
When the H-E staining of the control group of the MCF-7 cell line is examined, it is seen that the cells have epithelial morphology. In some areas, it is observed that the cells are concentrated and aggregated. In the linalool-treated group of the MCF-7 cell line, colony morphologies were observed to deteriorate due to a significant decrease in the number of cells (p < .001). Reduction in nuclear volume and hyperchromasia in the nuclear structure were commonly detected in cells (Figure 3). When the H-E staining of the control group belonging to the MDA-MB-231 cell line (no treatment was applied), round and spindle-shaped cell morphologies characterized by the cell line used was determined. Cell colonies were homogeneously dispersed at varying cell densities. In the linalool-treated group of the MDA-MB-231 cell line, the number of spindle-shaped cells decreased significantly compared to the control group (p < .001), while the number of round-shaped cells remained the same (p > .05). The decrease in the cytoplasm/nucleus volume ratio of the cells indicates the atrophic cell pattern. It was determined that the colony formation structures and the number of cells forming the colony were significantly decreased compared to the control group (p < .001) (Figure 4).
Improvement of chemosensitivity and inhibition of migration via targeting tumor epithelial-to-mesenchymal transition cells by ADH-1-modified liposomes
Published in Drug Delivery, 2018
Zhaoming Guo, Wenqing Li, Yue Yuan, Kun Zheng, Yu Tang, Kun Ma, Changhao Cui, Li Wang, Bing He, Qiang Zhang
MCF7 PTX-R cells were successfully established after continuous exposure to PTX for more than five months. Figure 2(A) showed the PTX-sensitivity assay of parental MCF7 and MCF7 PTX-R cells. The IC50 values of parental MCF7 and MCF-7 PTX-R cells were 77.7 nM and 320.7 nM, respectively. We could calculate the resistance index was 4.13. These results demonstrated that MCF7 PTX-R cells obtained PTX resistance. In addition, MCF7 PTX-R cells showed increased resistance to doxorubicin (Figure S1). MCF7 PTX-R cells displayed markedly different light-microscopic appearance compared with the parental cells (Figure 2(B)). The parental MCF7 cells had an epithelioid and paving stone appearance. In contrast, MCF-7 PTX-R cells adopted spindle-shaped or pear-like morphology and showed a decrease in cell–cell contacts. These changes were typical morphologic characteristics of the mesenchymal phenotype. Hence, MCF-7 PTX-R cells have transformed into the mesenchymal phenotype consistent with the morphologic characteristics of cells undergoing EMT (Yang et al., 2006; Nieto, 2009).
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