Molluscum contagiosum
Shiv Shanker Pareek in The Pictorial Atlas of Common Genito-Urinary Medicine, 2018
Molluscum contagiosum is usually self-limiting and symptoms may disappear in several months to about three years. Large, troublesome or persistent lesions may be treated as follows: Cryotherapy (liquid nitrogen) – to freeze and remove lesions.Trichloroacetic acid.Curettage – scraping lesions off the skin with a metal instrument.Diathermy – electrically produced heat treatment.Laser treatment.Imiquimod – topical immune-activating cream.
Superficial Peeling
Antonella Tosti, Maria Pia De Padova, Gabriella Fabbrocini, Kenneth R. Beer in Acne Scars, 2018
Suggestions for choice of peeling agent include: Trichloroacetic acid: Boxcar scars, especially in patients without active acne lesionsGlycolic acid: Macular scars, especially in patients without active acne lesionsPyruvic acid: Active acne lesions, macular lesions, and/or very superficial boxcar scarsSalicylic acid: Active acne lesions and/or macular scarsJessner's solution followed by trichloroacetic acid: Active acne lesions, superficial boxcar scars, and/or macular scars
Nail care and nail cosmetics
Archana Singal, Shekhar Neema, Piyush Kumar in Nail Disorders, 2019
Steps of the peel: Take informed consent and pre-peel photographs.Apply Vaseline on the cuticles and on nail folds.Clean the affected nail with alcohol/pre-peel cleanser.Apply single coat of the peel with nylon brush/ear bud.Ask the patient to wash hands after 10 minutes.Liberally moisturize the nails in between sessions.Repeat sessions at 2–4 week intervals.The peels most commonly used are medium-depth peels such as 70% glycolic acid, and a combination of 8% phenol with 15% trichloroacetic acid.
Effect of cabergoline alginate nanocomposite on the transgenic Drosophila melanogaster model of Parkinson’s disease
Published in Toxicology Mechanisms and Methods, 2018
Saba Khanam, Falaq Naz, Fahad Ali, Rahul Smita Jyoti, Ambreen Fatima, Wasi Khan, Braj Raj Singh, A. H. Naqvi, Yasir Hasan Siddique
The protein carbonyl content (PCC) was estimated according to the protocol described by Hawkins et al. (2009). The brain homogenate was diluted to a protein concentration of approx 1 mg/ml. About 250 μl of each diluted homogenate was taken in eppendorf centrifuge tubes separately. To it 250 μl of 10 mM 2,4-dinitrophenyl hydrazine (dissolved in 2.5 M HCl) was added, vortexed, and kept in dark for 20 min. About 125 μl of 50% (w/v) trichloroacetic acid was added, mixed thoroughly, and incubated at –20 °C for 15 min. The tubes were then centrifuged at 4 °C for 10 min at 9000 rpm. The supernatant was discarded and the pellet obtained was washed twice by ice-cold ethanol:ethyl acetate (1:1). Finally, the pellets were redissolved in 1 ml of 6 M guanidine hydrochloride and the absorbance was read at 370 nm.
The randomized trials of 10% urea cream and 0.025% tretinoin cream in the treatment of acanthosis nigricans
Published in Journal of Dermatological Treatment, 2021
Arucha Treesirichod, Suthida Chaithirayanon, Thitiwat Chaikul, Somboon Chansakulporn
Correction of the underlying cause is an ideal treatment of AN (8). However, goals in treating hyperpigmentation associated with AN are to decrease the skin pigmentation and to improve the skin texture and the patient’s quality of life. The efficacy of topical retinoids in the treatment of AN have been studied. The topical tretinoin and topical adapalene for childhood AN showed significant improvement in skin darkening (1,9). In an 8-week study of childhood AN, 0.1% adapalene and 0.025% tretinoin improved skin hyperpigmentation by 24.2 ± 7.9% and 23.8 ± 8.3%, respectively. The result from topical tretinoin treatment in children was better than in adults. Future research should explore a relationship between the result of treatment and age groups. Beside the retinoids, several treatment options have been proposed especially when treating the underlying diseases is not possible. These include other topicals such as calcipotriol and trichloroacetic acid peel (10–13). The oral treatment options also exist for AN. Oral retinoids are an effective treatment option for AN. However, adverse effects of systemic treatment should be considered (14–16). Metformin and rosiglitazone also showed a slight improvement of AN associated insulin resistance (17). Other treatments such as Alexandrite laser have been reported to be used in patients with AN (18,19).
LPS-induced Apoptosis is Partially Mediated by Hydrogen Sulphide in RAW 264.7 Murine Macrophages
Published in Immunological Investigations, 2019
Leema George, Tamizhselvi Ramasamy, KNS Sirajudeen, Venkatraman Manickam
DNA fragmentation was measured by the diphenylamine assay (Meβmer et al. 1996). Briefly, following incubations, cells were scraped off the culture plates, resuspended in 250 µl 10 mMTris, 1 mM EDTA, pH 8.0 (TE buffer), and incubated with an additional volume of lysis buffer (5 mM Tris, 20 mM EDTA, pH 8.0, 0.5% Triton X-100) for 30 min at 4°C. After lysis, the intact chromatin (pellet) was separated from DNA fragments (supernatant) by centrifugation for 15 min at 13,000 g. Pellets were resuspended in 500 μl Tris-EDTA (TE) buffer and samples were precipitated by adding 500 μl 10% trichloroacetic acid at 4°C. Samples were pelleted at 1400 g for 10 min and the supernatant was removed. After addition of 300 µl of 5% trichloroacetic acid, samples were boiled for 15 min. DNA contents were quantitated using the diphenylamine reagent.
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