Clinical Progresses in Regenerative Dentistry and Dental Tissue Engineering
Vincenzo Guarino, Marco Antonio Alvarez-Pérez in Current Advances in Oral and Craniofacial Tissue Engineering, 2020
Revascularization studies have established the following prerequisites: Revascularization occurs most predictably in teeth with open apices and necrotic pulp secondary to trauma.Apex is open more than 1 5 mmBacteria should be removed from the canal by any of the following methods: 3 mix—MP triple antibiotic paste consisting of ciprofloxacin, metronidazole and minocycline.Calcium hydroxide, formocresol.Effective control seal.Matrix into which new tissue can grow.Patient should beUse of anesthetic without a vasoconstrictor when trying to induce bleeding.No instrumentation of canalsSodium hypochlorite is used as an irrigant.Formation of blood clot probably serves as a protein scaffold permitting 3-dimensional in growth of tissue.
Pulp Therapy for Primary Teeth
M S Duggal, M E J Curzon, S A Fayle, K J Toumba, A J Robertson in Restorative Techniques in Paediatric Dentistry, 2021
Since the 1950s, much work has been done to evaluate the effect of formocresol on pulp tissue, including histological, biochemical and histochemi-cal enzymatic studies. Essentially, formocresol acts through the aldehyde group of formaldehyde, forming bonds with the side-groups of the amino acids of both the bacterial proteins and those of the remaining pulp tissue. It is therefore both a bactericidal and devitalizing agent. It kills off and converts bacteria and pulp tissue into inert
Efficacy of platelet concentrates in pulpotomy – a systematic review
Published in Platelets, 2018
Roshan Noor Mohamed, Sakeenabi Basha, Yousef Al-Thomali
Recent treatment approaches to pulpal infection or injury include the traditional root canal treatment with the use of improvised synthetic materials, which provide a better pulpal seal compared to CH or biological approach based on molecular and cellular basis for pulpal regeneration (2–5,9,11). Pulpotomy treatment for the primary or permanent teeth includes devitalization, preservation, or regeneration (5–8). Devitalization is done mainly in primary tooth by formocresol, electrosurgery, or by laser technique which destroys or mumifies the vital tissues (34–36). Preservation is done using zinc oxide eugenol, glutaraldehyde, or ferric sulfate pulpotomy, which maintains the maximum vital tissue with no induction of reparative dentin (34–37). Regeneration and formation of dentin bridge are done by CH (11), MTA (9,11), bone morphogenetic proteins, or growth factors (38). Platelet concentrates, an autologous derivative with a higher platelet concentration than baseline, is the material of choice for regeneration as it contains high growth factors and it forms fibrin matrix, which in turn holds growth factors in it. The present systematic review was conducted to analyze the efficacy of platelet concentrates in pulpotomy of primary or permanent teeth in humans. Four studies were included in the present review (26,27,30,31).
Which procedures and materials could be applied for full pulpotomy in permanent mature teeth? A systematic review
Published in Acta Odontologica Scandinavica, 2019
M. Zanini, M. Hennequin, PY. Cousson
The flow chart for study inclusion is presented in Figure 1. One single investigator (MZ) conducted the research on databases and for backward research. All three authors consensually agreed for abstracts to be excluded during the screening phase. During this screening phase, the criteria for exclusion were: not in English; in vitro studies; animal studies, reviews; comments; articles reporting pulpotomies on primary teeth, immature permanent teeth, or dens invaginatus; studies on vital direct pulp capping, partial excavation or partial pulpotomy; papers related to general issues in paediatric dentistry; studies related to the use of formocresol; articles retracted by the journal’s editor.
Coexistence of Candida albicans and Enterococcus faecalis increases biofilm virulence and periapical lesions in rats
Published in Biofouling, 2021
Qian Du, Shasha Yuan, Shuangyuan Zhao, Di Fu, Yifei Chen, Yuan Zhou, Yangpei Cao, Yuan Gao, Xin Xu, Xuedong Zhou, Jinzhi He
40 Sprague Dawley rats (male, 8 weeks old, 200 ∼ 250 g) were housed in specific pathogen-free animal facility. The surgical procedures were conducted in accordance with Lu et al. (2015) with minor modifications. Briefly, after general anesthesia, the pulps of the rat upper left first molars were exposed by using a quarter size round bur (Dentsply Maillefer, Ballaigues, Switzerland). The pulp was left exposed to the oral environment for three weeks to develop the periapical periodontitis lesions. The rats were anesthetized again. The distal root canals of the upper left first molars were instrumented with reamers and files (Dentsply Maillefer, Ballaigues, Switzerland), along with copious irrigation of 15% EDTA (Xilong Chemical Co, Ltd, Guangdong, China) and 2.5% NaOCl (Ziyi Reagent Factory, Shanghai, China). After root canal preparation, a cotton pellet soaked with formocresol (Second Medical Zhangjiang Biological Material Co, Ltd, Shanghai, China) was placed into each pulp cavity, then the access cavity was sealed with glass ionomer cement (ShoFu, Kyoto, Japan). Two weeks later, the rats were put under anesthesia again. The sealing material and cotton pellets were removed from pulp cavity. The root canals were irrigated with 1 ml saline and dried with sterile paper points. Rats (n = 10 per group) were randomly selected into 4 groups. The pulp cavity of each rat was infected with (1) C. albicans, (2) E. faecalis, (3) C. albicans + E. faecalis, or (4) PBS as a sham control, 0.1 ml for each group. The concentration of E. faecalis and C. albicans was 1 × 107 CFU ml−1 and 1 × 103 cells ml−1, respectively. The access cavity was then sealed with glass ionomer cement, and another two weeks later, the rats were sacrificed by CO2 inhalation.
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