The Processes of Rejection and Nonrejection — Histology and Functional Competence of Rat Livers After Transplantation
Naoshi Kamada in Experimental Liver Transplantation, 2019
Syngeneic controls were included DA liver into DA recipients and PVG liver into PVG recipients. The results showed that the nonrejection of DA liver by PVG and F1 recipients was not due to a failure of the host to initiate an anti-graft response, but rather to the termination of the rejection reaction at an early stage in PVG and at a more advanced stage in the F1, hybrid. Biopsy specimens from grafted livers were taken at laparotomy and immediately fixed in buffered 10% formalin. The tissues were embedded in paraffin and sections were stained with hematoxylin and eosin. DA liver transplanted orthotopically into BN recipients was acutely rejected in seven out of eight rats, with one animal showing chronic rejection. In acute rejection, there is marked swelling of portal tracts caused mainly by edema and cellular infiltration. The cells are mononuclear and mostly of histiocytic origin, with occasional lymphocytes and large pyroninophilic cells.
Retrospective Diagnosis: Pam and Gae
A. Julio Martinez in Free-Living Amebas: Natural History, Prevention, Diagnosis, Pathology, and Treatment of Disease, 2019
Retrospective diagnoses of free-living amebic infections have been made while examining sections from central nervous system tissues fixed in formalin, embedded in paraffin, and stained with hematoxylin and eosin. The histologic material should be formalin-fixed to ensure the best results. If tissues are fixed in mercury fixatives, they cannot be stained by the Immunofluorescent Antibody Technique (IFAT) unless the mercury is first removed with alcohol-iodine; even then the fluorescence is weaker than if the same tissue had been fixed in formalin. The common histologic sections are used to perform the IFAT after deparaffinization of the tissues. In addition to its value in clinical diagnosis, IFAT provides a rapid screening method for the detection of pathogenic free-living amebas in swimming pools, tap water, and other domestic and recreational water supplies. The Immunoperoxidase Method is based on the use of the enzyme horseradish peroxidase as a marker to visualize antigens at the cellular surface level.
Rhabdomyosarcoma
Dongyou Liu in Tumors and Cancers, 2017
Skeletal muscle tumors are subdivided into benign (rhabdomyoma [adult type, fetal type, and genital type]) and malignant (embryonal rhabdomyosarcoma [RMS]), alveolar RMS, spindle cell or sclerosing RMS, and anaplastic or pleomorphic RMS. Hard and soft tissues are the key components of the musculoskeletal system. RMS is soft tissue sarcoma (STS) that arises from primitive mesenchymal cells/myogenic precursor cells and demonstrates evidence of skeletal muscle differentiation. Depending on the location, clinical symptoms of RMS include persistent lump or swelling; fever; headache; nausea; vomiting; abdominal pain; constipation; trouble urinating; blood in the urine; bleeding from the nose, throat, vagina, or rectum; bulging or swollen eye (proptosis) (orbital RMS, about 10 percent of cases); painless scrotal mass (paratesticular tumor); protruding grapelike mass in the vagina (botryoidal RMS); weakness; and weight loss. Diagnosis and identification of RMS subtypes rely on hematoxylin and eosin (H&E), immunohistochemistry, and/or electron microscopy. Treatment options for RMS consist of surgery, radiotherapy, and chemotherapy.
Foreskin Analysis of Circumcised Boys With and Without Previous Topical Corticosteroid
Published in Fetal and Pediatric Pathology, 2012
Luis Gustavo Sabino Borges, Adriana Cartafina Perez-Bóscollo, Laura Penna Rocha, Renata Calciolari Rossi Silva, Camila Souza de Oliveira Guimarães, Eumenia Costa da Cunha Castro, Rosana Rosa Miranda Corrêa
Problems with the foreskin are common reasons for pediatric surgery consultations. We collected the foreskin of 40 patients for 2 years and these samples were divided into groups with and without previous topical corticosteroid. We carried out histochemical hematoxylin & eosin and Picrosirius analyses of the foreskin. Collagen fibers and inflammatory infiltrate was higher in samples from patients who had complications related to phimosis. Fibrosis was higher in patients who used topical corticosteroid. A histopathologic study of the foreskin may provide an additional analysis of patients undergoing circumcision and it can also improve the accuracy of surgical indication.
How to Study Boron Biodistribution in Liver Metastases from Colorectal Cancer
Published in Journal of Chemotherapy, 2004
L. Roveda, U. Prati, J. Bakeine, F. Trotta, P. Marotta, P. Valsecchi, A. Zonta, R. Nano, A. Facoetti, P. Chiari, S. Barni, T. Pinelli, S. Altieri, A. Braghieri, P. Bruschi, F. Fossati, P. Pedroni
Summary The purpose of this study was to evaluate boron distribution for a safe and effective BNCT (Boron Neutron Capture Therapy) of liver metastases. Samples both from healthy and tumour liver parenchyma were analysed, after i.v. boron administration, by: a particles counting under neutron irradiation; morphological analysis by standard haematoxylin-eosin staining; neutron autoradiography. Our method was unaffected by the cytological heterogeneity inside tumour nodules; it demonstrated selective boron distribution in tumour tissue and predicted estimated mean therapeutic doses in tumour and safety doses in healthy tissue. The time interval for efficient BNCT was 2 to 4 hours after i.v. boron administration.
Morphologic analysis of fetal stress organsin different causes of perinatal death
Published in Fetal and Pediatric Pathology, 2012
Rosana Rosa Miranda Corrêa, Ana Paula Espíndula, Renata Calciolari Rossi e Silva, João Carlos Saldanha, Camila Souza de Oliveira Guimarães, Camila Lourencini Cavellani, Marlene Antônia dos Reis, Vicente de Paula Antunes Teixeira, Eumenia Costa da Cunha Castro
Complications act as stress-inducers during pregnancy so the fetus can develop functional compensatory mechanisms or morphologic changes. The cases analyzed are with congenital malformations or acute stress; chronic included cases with ascending infection (AI) and perinatal hypoxia/anoxia (PHA). The hematoxylin-eosin (H&E) was done to analyze the vacuolization, and the immunohistochemistry to the phagocytosis. The discreet standard of vacuolization was observed in 52.6% of the cases, 22.1% moderate, and 25.3% severe. The number of macrophages was higher in PHA. Changes in these organs are closely related to the cause of death and to the period during which the harmful agent.
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