Overview of HIV Infection
Mark J. Rosen, James M. Beck in Human Immunodeficiency Virus and the Lung, 1998
or Giemsa staining, tachyzoites can be seen in alveolar lining cells (5). The best results have been reported with the use of eosin-methylene blue fast staining (5,24) or Giemsa staining (5,24,25) of bronchoalveolar lavage fluid (BAL). In one series, BAL was diagnostic in 88% of patients with pulmonary toxoplasmosis (16). In the study by Oksenhendler et al., BAL was diagnostic in 10 of 11 patients (6). However, the yield of BAL may be lower with infection that is not severe. Derouin et al. recently proposed that tissue culture on human embryonic fibroblast cell line MRC5 (Bio Merieux, Lyon, France) provided evidence of infection within 2 days of inoculation in patients in whom diagnosis was not made by stained smears (24). In another series, the diagnosis was also made by observation of parasites in
Miscellaneous Respiratory Infections
Adam T. Hill, F. X. Emmanuel, W.H.B. Wallace in Pulmonary Infection, 2004
Sputum, pus, or bronchoalveolar lavage or biopsy material are examined for characteristic endosporing spherules by microscopy, using wet microscopy, methenamine silver stain, and eosin-methylene blue stain. Hyphal forms may be seen in cavitating lesions. Figure 7.7 is a photomicrograph of a lung section from a patient who had lived in Arizona who presented with cavitating lung lesions. The section is silver stained (Grocott) and shows rounded spores and also small nonbranching hyphae, the appearances of which would be consistent with coccidiomycosis.
Escherichia
Dongyou Liu in Laboratory Models for Foodborne Infections, 2017
E. coli is a rod-shaped bacterium of about 0.6 μm in diameter and 2 μm in length. The bacterium forms nonspreading black colonies with a characteristic greenish-black metallic sheen on eosin methylene blue (EMB) agar, and deep red colonies on MacConkey agar. Other morphological features of note include:
Genomic characterization of four Escherichia coli strains isolated from oral lichen planus biopsies
Published in Journal of Oral Microbiology, 2021
Huitae Min, Keumjin Baek, Ahreum Lee, Yeong-Jae Seok, Youngnim Choi
Although there is a possibility that E. coli colonizing the oral mucosa of healthy subjects are genetically different from the OLP-isolated strains, the OLP strains are more likely to be commensals but increase virulence in the altered environment of OLP patients. Isolation of E. coli strains from the oral mucosa of healthy individuals will clarify this issue. Despite the presence of lacZ, lacY, and lacA genes, the OLP-isolated strains did not form purple-black colonies on a selective and differential medium, such as eosin methylene blue agar. Because E. coli was substantially enriched within the OLP tissues (up to 60%), it was possible to isolate E. coli without colour differentiation. In the buccal swabs of healthy subjects where E. coli accounts for 0.03–7% of total bacteria, however, other Gram-negative species such as Haemophilus spp., Neisseria spp., and Lautropia mirabilis, but not E. coli, have been isolated from cultures on the eosin methylene blue agar.
Bacterial contamination of coins obtained from school canteen and green market
Published in Infectious Diseases, 2019
Mustafa Yontem, Sabire A. Doyuk, Fatih Erci, Behiç S. Erdogdu
The study was carried out in Eskisehir, Turkey. In the study, 50 money coins were collected from school canteens and different vendors of the green market by random sampling method. Of the money samples, 25 were taken from school canteens, 25 from different vendors in the green market, and the collected money was divided into four groups. Each of the coin samples was supplied with sterile plastic bags from the direct source without any contact, and each bag was placed in a clean plastic box. The collection of the money samples was completed for a total of three hours and was brought to the laboratory for analysis on the same day. The collected coins were transferred into 15 ml of liquid Brain-Heart Infusion Broth and shaken at 2000 rpm for 15 min. About 100 μL from each sample inoculated on Blood and Eosin Methylene Blue agar medium. The samples incubated at 37 °C for 48 h were stained with Gram stain for morphological preliminary evaluation and separated according to morphological structures.
In vivo toxicity evaluation of nanoemulsions for drug delivery
Published in Drug and Chemical Toxicology, 2021
Mariana Appel Hort, Barbara da Silva Alves, Osmar Vieira Ramires Júnior, Mariana Correa Falkembach, Gabriela de Moraes Soares Araújo, Caroline Lopes Feijo Fernandes, Ronan Adler Tavella, Juliana Bidone, Cristiana Lima Dora, Flavio Manoel Rodrigues da Silva Júnior
The MN assay was performed according to OECD (2014) and da Silva-Júnior et al. (2013). Bone marrow cells were obtained from femurs immediately following rat euthanasia. Smear preparations were made on slides, air dried, and stained with eosin methylene blue according to Leishman. At least two slides with cells from each animal were prepared. The proportion of polychromatic (PCE) and normochromatic (NCE) erythrocytes was determined for each animal by counting the NCEs in at least 200 PCEs. The frequency of micronucleated cells was analyzed in 1000 PCEs per animal; the results show the average of both slides (micronucleated cells in 1000 PCEs).
Related Knowledge Centers
- Coliform Bacteria
- Escherichia Coli
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- Eosin
- Staining
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- Gram-Positive Bacteria
- Growth Medium
- Lactose
- Dipotassium Phosphate