Aquatic Plants Native to Europe
Namrita Lall in Aquatic Plants, 2020
When different extracts of the plant were tested against 26 strains of bacteria and fungi, it was reported that the activity varied depending on the type of plant extract and on the group of microorganisms (Radojevic et al. 2016). It has been reported that the ethanolic extract of the plant had high activity against Proteus mirabilis, even higher than that of the positive control (tetracycline). In general, Gram-positive bacteria were more sensitive to the extracts of the plant. The antifungal activity of the plant extracts was relatively low, except for the activity against Aspergillus restrictus, where the minimum inhibitory concentrations were found to be better than the control (fluconazole). Another report demonstrated the antimicrobial activity of the plant against Pseudomonas putida, P. morganii, P. mirabilis, and K. pneumoniae (Parekh and Chanda 2007). It has been mentioned that when different solvents were used for the antimicrobial tests, the activity increased with increasing polarity of the solvent.
Quorum Sensing and Essential Oils
K. Hüsnü Can Başer, Gerhard Buchbauer in Handbook of Essential Oils, 2020
Jaramillo-Colorado et al. (2012) investigated the anti-QS activity of different Columbian plants on a Pseudomonas putida, as well as on an E. coli, strain. As the chemical composition of Lippia alba oils depends on the collection location of the plants, tests were carried out with oils from different collection sites. Additionally, the team differed between oils extracted by means of microwave-assisted hydro-distillation (MWHD) or hydro-distillation (HD). In concentrations of 1.2 mg/mL, anti-QS activity was measured for some of the L. alba oils, Ocotea sp., and Elettaria cardamomum in P. putida (the highest activity inhibition was reached by a the geranial/neral chemotype of L. alba), whereas only two of the limonene/carvone chemotypes showed a low inhibitory activity of 9% and 1%, respectively). All of the oils were active QS inhibitors in E. coli, the most active was an oil of L. alba chemotype 2 and Ocotea sp. (Jaramillo-Colorado et al., 2012).
Physiology of Moss-Bacterial Associations
R. N. Chopra, Satish C. Bhatla in Bryophyte Development: Physiology and Biochemistry, 2019
Bacteria and possibly algae and other fungi play a significant role in the growth and fruiting process of mushrooms. Agaricus brunnescens, which fails to fruit on sterilized substrates, does so when Pseudomonas putida is present.48 The growth and development of both the fungal mycelia and the bacteria are stimulated by extracts from the other. Not all strains of P. putida cause fruiting bodies to form in A. brunnescens, nor do all mushrooms respond similarly to this bacterium. Other microorganisms implicated in the phenomenon of fungal fruiting are Bacillus megaterium, Arthrobacter terregens, and Rhizobium meliloti, as well as yeasts and microalgae.49,50 The removal of inhibitors, the production of growth factors, or the alteration of gene transcription through surface interactions may play a still to be documented role in these responses.51
Recovery of Biosurfactant Using Different Extraction Solvent by Rhizospheric Bacteria Isolated from Rice-husk and Poultry Waste Biochar Amended Soil
Published in Egyptian Journal of Basic and Applied Sciences, 2020
S. O. Adebajo, P. O. Akintokun, A. E. Ojo, A.K. Akintokun, O.A. Badmos
The study shows that efficient biosurfactant producers could be recovered from biochar-amended soil of plant (rice-husk) or animal (poultry waste) sources. The result also revealed that rice-husk waste biochar-amended soil harbors efficient biosurfactant producers and effective hydrocarbon degrader. Pseudomonas putida, a gram-negative, rod-shaped bacterium that belongs to the genus of Pseudomonas has proven to be a rhizospheric potent biosurfactant producer, able to produce biosurfactant with an environmental waste (palm oil mill effluent) as a substrate using different recovery solvents. The crude biosurfactant produced has moderate stability and antimicrobial property especially on gram positive bacteria. These finding also suggested the drop collapse method as a suitable primary screening method.
Three cases of retained cuff related infection after manual pull removal of peritoneal dialysis catheter
Published in Renal Failure, 2021
Suojian Zhang, Xu Zhang, Haitao Li, Zhiqiang Wei, Juan Cao
A 49-year-old man was initiated on PD treatment 6 years ago, and the primary causative disease was chronic glomerulonephritis. Owing to peritoneal ultrafiltration failure, PD treatment was stopped 6 months ago. PD catheter was removed by the ‘pull technique’. After 1 month, fluid exudation was noted at the exit site of the original PD catheter, accompanied by pain and no fever. Blood test results revealed the following: hemoglobin 118 g/L, white blood cell count 6.42 × 109/L, neutrophil percentage 78.5%, platelet count 166 × 109/L, C-reactive protein 2.56 mg/L, and procalcitonin 0.67 ng/mL. Bacterial culture of the secretion revealed the presence of Pseudomonas putida. B-mode ultrasound imaging revealed a heterogeneous echo mass in the left lower abdominal wall, measuring approximately 38 × 7 mm (Figure 2). We first administered piperacillin–sulbactam 2.5 g IV BID for 8 days without mass removal; however, the patient did not show any improvement, and B-mode ultrasound imaging indicated that the size of the mass in the left lower abdominal wall increased to approximately 45 × 8 mm (Figure 3). Abdominal wall mass resection was performed, and piperacillin–sulbactam was administered for 1 more week. Subsequently, the patient was cured.
Continuous flow system for biofilm formation using controlled concentrations of Pseudomonas putida from chicken carcass and coupled to electrochemical impedance detection
Published in Biofouling, 2020
Daoyuan Yang, José I. Reyes-De-Corcuera
Pseudomonas putida, a widely used model organism for biofilm formation (Klausen et al. 2006; Giaouris et al. 2013), was selected as a single culture to show proof of concept of the ability of the system to detect biofilm formation. Specifically, P. putida #8 from the University of Georgia’s Food Science and Technology culture collection, isolated from poultry carcass was used for this research. One frozen bead of P. putida was thawed and inoculated into 9 ml of tryptic soy broth (TSB) to grow overnight at 28 °C. Tryptic soy agar (TSA) plates were streaked with the P. putida suspension and incubated at 28 °C overnight to make cultures. Cultures were stored at 4 °C and renewed every three months. A loop of bacteria from the prepared culture was transferred into 200 ml 1/10 TSB and incubated at 28 °C with agitation (160 rpm, G24 Environment Incubator Shaker, New Brunswick Scientific, Enfield, CT) for 24 h.
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