Order Caudovirales
Paul Pumpens, Peter Pushko, Philippe Le Mercier in Virus-Like Particles, 2022
The phage λ-based display was used for the cell targeting. Thus, the cancer cells were targeted by the display of high-affinity nanobody against HER2 (Shoae-Hassani et al. 2013). The human 293T cell line was targeted by the display of chemically coupled human holotransferrin, and delivery of the GFP-encoding gene was realized (Khalaj-Kondori et al. 2011). The mammalian cell lines were also targeted by display of the following addressing tools: the RGD motif (Dunn 1996); TAT transduction domain of HIV-1 (Eguchi et al. 2001; Wadia et al. 2013); single-chain antibodies (Gupta et al. 2003) and single-chain anti-CEA antibody (Vaccaro et al. 2006); the full-length adenoviral penton base or its central domain of aa 286–393 (Piersanti et al. 2004); ανβ3 integrin-binding peptide (Zanghi et al. 2005) together with delivery of luciferase gene (Lankes et al. 2007); ubiquitinylation and CD-40 binding motifs (Zanghi et al. 2007); FcγRI (Sapinoro et al. 2008). The Cry1Ac toxin was displayed to address the VLPs to the receptor of Bacillus thuringiensis insecticidal toxin (Vílchez et al. 2004).
Effect of bacterial toxin identified from the Bacillus subtilis against the Cnaphalocrocis medinalis Guenée (Lepidoptera: Crambidae)
Published in Toxin Reviews, 2023
Ramakrishnan Ramasubramanian, Sengodan Karthi, Sengottayan Senthil-Nathan, Haridoss Sivanesh, Narayanan Shyam Sundar, Vethamonickam Stanley-Raja, Govindaraju Ramkumar, Kanagaraj Muthu-Pandian Chanthini, Prabhakaran Vasantha-Srinivasan, Khaloud Mohammed Alarjani, Mohamed S Elshikh, Ahmed Abdel-Megeed, Patcharin Krutmuang
The probable insecticidal action of crystal proteins expressed by the particular crystal genes working against the lepidopteran pest has been reported to be cry1Aa, cry1Ab, cry1Ac, cry1B and cry1C.41 However, among numerous Bt crystal protein genes, the cry1C, cry1D, cry1E and cry1F genes have revealed insecticidal activity against Spodoptera spp. (Valicente et al. 2010). These results could be the reason that the Bacillus strain corresponding with cry1Aa, cry1Ab and cry1Ac, whose genes show significant toxicity against C. medinalis.
Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac
Published in Journal of Microencapsulation, 2021
Yongjing Zhang, Aijing Zhang, Mengyuan Li, Kanglai He, Shuyuan Guo
The FC without UV irradiation had an obvious band at 130 kDa (Figure 2(a), lane 2). The protein amount remained 53.39 ± 1.93% after UV radiation for 2 h, while the amount of protein decreased significantly with the increase of UV irradiation time (Figure 2(a)). In Figure 2(b), we found that the crystal protein could not be dissolved in the alkaline solution after UV irradiation for more than 4 h, which indicated that the physical and chemical properties of Cry1Ac had changed. Consequently, Cry1Ac could not play the insecticidal activity after irradiation for more than 4 h.
Related Knowledge Centers
- Bacillus Thuringiensis
- Genetic Engineering
- Immunologic Adjuvant
- Insecticide
- Mucus
- Spore
- Toxication
- Gram-Positive Bacteria
- Delta Endotoxin
- Gene