Next Generation Tissue Engineering Strategies by Combination of Organoid Formation and 3D Bioprinting
Naznin Sultana, Sanchita Bandyopadhyay-Ghosh, Chin Fhong Soon in Tissue Engineering Strategies for Organ Regeneration, 2020
3D Bioprinting has evolved dramatically during the last decade as an approach that is at the crossroad of bioengineering and regenerative medicine. It offers humongous potential to design patient-specific and tissue/organ specific tissue engineered equivalents. The advent of rapid manufacturing technologies associated with the engineering aspect of bioprinting offers precise control over architecture and resolution, which makes this field way ahead of the current tissue engineering and regenerative medicine approaches (Cui et al. 2017). Bioprinting offers the possibility to guide the specific orientation of the encapsulated cells, thus effectively recapitulating the tissue/ organ specific microstructure, which in turn simulates the biological, mechanical and functional properties of the target tissue/organ. The promising potential of the field can be delineated from the fact that even in 2D culture systems micro-patterning based arrangement of cells lead to self-assembled gene expression patterns similar to early embryonic organization events (Warmflash et al. 2014). Morgani et al. reported similar results: mouse pluripotent stem cells cultured on micropatterned surfaces under the influence of specific morphogens could mimic embryonic spatial patterning akin to in vivo regionalized pattering of embryo (Morgani et al. 2018). It would be highly interesting if such approaches can be combined with 3D bioprinting to recapitulate fundamental pattern formation features of developmental stages.
From Conventional Approaches to Sol-gel Chemistry and Strategies for the Design of 3D Additive Manufactured Scaffolds for Craniofacial Tissue Engineering
Vincenzo Guarino, Marco Antonio Alvarez-Pérez in Current Advances in Oral and Craniofacial Tissue Engineering, 2020
However, in this context 3D cell printing technology would also be an interesting approach enabling researchers to suspend and position cells embedded in materials such as hydrogels (Tao et al. 2019). 3D bioprinting could allow in obtaining specific mechanical properties, cell interactions and desired distribution of growth factors. The possibility of printing blood capillaries has been already reported and cell printing for craniofacial tissue regeneration would seem feasible, even if many studies are still ongoing (Tao et al. 2019).
The Future Is Not What It Used to Be
Tom Lawry in Hacking Healthcare, 2022
Bioprinting (also known as 3D bioprinting) combines 3D printing with biomaterials to replicate parts that imitate natural tissues, bones, and blood vessels in the body. It is mainly used today in connection with drug research. It also has been used recently to create “cell scaffolds” to help repair damaged ligaments and joints.
Current advances in cell therapeutics: a biomacromolecules application perspective
Published in Expert Opinion on Drug Delivery, 2022
Samson A. Adeyemi, Yahya E. Choonara
Interestingly, several clinical trials in which cellulose sulphate is used as the biomacromolecule for encapsulation, the microcapsules showed viable anti-tumour effects and exhibited superior biocompatibility with no adverse reactions such as a host immune response. Further research is needed to understand the role of various biomacromolecules in mounting an immune response. With the significant advances made in complementary scientific domains such as nanotechnology and 3D-bioprinting, the future of smart implantable cell therapeutics is closer than expected. 3D-bioprinting is a rapidly growing technology that holds great potential for the design and fabrication of functional tissues and organs. Using this approach, the patient<apos;>s cells can be utilized to design, engineer and produce constructs to replace diseased tissues and organs.
Tackling pharmacological response heterogeneity by PBPK modeling to advance precision medicine productivity of nanotechnology and genomics therapeutics
Published in Expert Review of Precision Medicine and Drug Development, 2019
Ioannis S. Vizirianakis, Androulla N. Miliotou, George A. Mystridis, Eleftherios G. Andriotis, Ioannis I. Andreadis, Lefkothea C. Papadopoulou, Dimitrios G. Fatouros
Looking into the framework of the biomaterials used in 3D bioprinting, a diverse group of materials is available. The biocompatibility of these materials is of paramount importance, for cell growing and reproduction without causing any adverse reactions, like inflammation. Another critical parameter is the porosity of these biomaterials, which might affect the transport of essential elements and metabolism products into and from the interior of the structure respectively. Porosity also might affect the organization and the behavior of the cells, as the mechanical features of the materials (e.g., strength) are vital, considering bones or cartilages. Hydrophilicity, pH and non-toxic degeneration of the proposed materials are of great importance as well [50]. Equally important is the viability and maintenance of the cells following the printing process, whereas the properties of the materials, when used with stem cells, should guarantee the ability to retain their activity, including proliferation and pluripotency. Another key point in bioprinting relates to extracellular microenvironment creation by mimicking physiology, which must be adjusted in a way that the necessary stimuli for the cell functions can be regulated. A detailed description of the applications in 3D bioprinting in the pharmaceutics, the limitations of the process and the future perspective of this field are detailed by Mandrycky et al. [51] and Peng et al. [52], accordingly.
Nanofibers as drug-delivery systems for infection control in dentistry
Published in Expert Opinion on Drug Delivery, 2020
Maurício G. C. Sousa, Mariana R. Maximiano, Rosiane A. Costa, Taia M. B. Rezende, Octávio L. Franco
Thus, nanotechnology may offer endless possibilities for dentistry. In this context, nanofibers are flexible materials and can carry diverse antimicrobial molecules, as well as other nanostructures. Besides, electrospinning has established itself as the most effective and widespread method to produce nanofibers. This technique can contribute to the expansion of new 3D bioprinting possibilities. Also, the diversity of polymers used opens gaps for the discussion of cost, degradation, hydrophobicity, and interaction with the incorporated antimicrobial molecules. Given this, each area of dentistry presents specific demands that may facilitate or hinder its implementation. Thus, in the face of discussions about the barriers to the development of in vivo experiments, new 3D models of tissues present in the oral cavity may appear as options for improving in vitro tests and bringing them closer to the clinical reality.