Amylase
Masahiko Mori in Histochemistry of the Salivary Glands, 2019
Amylase in animals and human beings is mainly confined to the salivary glands and pancreas; however, the types of amylase are different in different organs and in different animal species. Immunohistochemical identification of α-amylase in tissue sections has been obtained using antiserum purified α-amylase isolated from different tissues and organs. Immunohistochemically detectable α-amylase was confined strictly to serous acinar compartments in the parotid and submandibular glands, and serous demilune cells in the sublingual gland. The α-amylase staining varied in both the serous acinar units or individual acinar cells. When obstructive adenitis occurred, the remaining acinar elements may produce α-amylase at irregular levels. Luminal materials in cystic dilated lesions also contained elevated α-amylase activity. Typical histologic structures of salivary pleomorphic adenoma showed positive α-amylase staining in most luminal cells of tubulo-ductal and cystic structures, whereas outer cells of probable myoepithelial origin were unstained.
Morphological differences in innervation between mucous glands and serous glands: a quantitative histological study using the sublingual glands of elderly humans
Published in Acta Oto-Laryngologica, 2015
Ai Kawamoto, Kei Kitamura, Masahito Yamamoto, Gen Murakami, Shin-ichi Abe, Yukio Katori
Conclusion: In the sublingual gland, the serous lobule usually carried a higher density of NSE-positive nerve elements than the mucous lobule, whereas the mucous acinus in the mucous lobule was larger than the serous acinus in the serous lobule. Objectives: To demonstrate quantitative differences in nerve elements between the mucous and serous lobules of sublingual glands. Methods: This study investigated using specimens from 14 donated cadavers (mean age = 78 years). Since immunohistochemistry for neuron-specific enolase (NSE) stains all nerves in addition to other mesenchymal cells possibly of nerve origin, the present quantitative evaluation was based on NSE-positive areas per visual field under a ×20 objective lens (0.6 × 0.45 mm when printed). Results: In mucous lobules, the areas occupied by NSE-positive nerve elements ranged from 5798–16,541 μm2 (mean ± SD = 9280 ± 2584 μm2). In contrast, the corresponding areas in serous lobules ranged from 7853–23,540 μm2 (mean ± SD = 13,520 ± 4351 μm2). The difference in NSE-positive areas was statistically significant (p = 0.0022). However, the mucous acinus in the mucous lobule was 2-times larger than the serous acinus in the serous lobule (2474 ± 1477 μm2 vs 1119 ± 632 μm2).
Surgical results of the intraoral approach for plunging ranula
Published in Acta Oto-Laryngologica, 2014
Conclusions: The intraoral approach for removal of the plunging ranula is a safe and effective surgical approach as a primary treatment modality for plunging ranula. Objectives: Although sclerotherapy is applied for plunging ranula, it can be primarily treated with surgery such as marsupialization, simple excision, and transcervical excision. This study was performed to assess the results of the intraoral approach for the treatment of plunging ranula. Methods: This prospective clinical study comprised a total of 23 patients with plunging ranula treated by the intraoral approach. Results: All patients had complete removal of the sublingual gland with evacuation of cystic fluid. The cystic wall of the ranula could be dissected completely in only four patients, and in eight patients it was only possible to drain the cystic fluid. However, in 11 patients we could not dissect the cystic wall completely. Rupture of ranula developed in all patients during the intraoral dissection. Recurrence was not detected in any of the patients during the median follow-up period of 14 months after the intraoral excision. There were no complications or external scarring.
Salivary gland cancer in Finland 1991–96: an evaluation of 237 cases
Published in Acta Oto-Laryngologica, 2005
Heikki Luukkaa, Pekka Klemi, Ilmo Leivo, Petri Koivunen, Jussi Laranne, Antti Mäkitie, Jukka Virtaniemi, Susanna Hinkka, Reidar Grénman
Conclusion In this material consisting of various salivary gland carcinomas, stage I, male gender and age were the most powerful predictors of patient outcome. Objectives To retrieve the records of all salivary gland cancer (SGC) patients diagnosed in Finland between 1991 and 1996 and to evaluate the incidence, histological type and location of SGC, the treatment given and the outcome. Material and methods The records for all SGCs (n=286) diagnosed in Finland between 1991 and 1996 and reported to the Finnish Cancer Registry were retrieved. The histological re-evaluation and retrospective study involved 237 SGC patients. Results The study population consisted of 125 males and 112 females. The mean age was 59 years (males 61 years, females 58 years). Follow-up was at least 5 years. The commonest tumor location was the parotid gland (n=152; 64%), followed by the minor salivary glands (n=46; 19%), the submandibular gland (n=38; 16%) and the sublingual gland (n=1; 0.4%). The most frequent histological types of SGC were adenoid cystic carcinoma (n=65; 27%), mucoepidermoid carcinoma (n=45; 19%) and acinic cell carcinoma (n=41; 17%). Surgery, either alone or in combination with other treatment modalities, was used in 209 cases (88%). Radiotherapy was given to 136 patients (57%), 13 of whom (5%) did not undergo surgery. The 5-year overall survival rate was 56.5%, and for stages I–IV it was 78%, 25%, 21% and 23%, respectively (p
Related Knowledge Centers
- Mouth
- Tongue
- Submandibular Gland
- Parotid Gland
- Salivary Gland
- Salivary Glands
- Saliva