Clinical Progresses in Regenerative Dentistry and Dental Tissue Engineering
Vincenzo Guarino, Marco Antonio Alvarez-Pérez in Current Advances in Oral and Craniofacial Tissue Engineering, 2020
Dental follicle is a loose vascular connective tissue composed of a heterogenous layer of an ectomesenchymal cells surrounding the enamel organ and the dental papilla of the developing tooth germ in early stages of tooth development prior to eruption (Lesot et al. 1993). This tissue contains progenitor cells that form the periodontium, i.e., cementum, PDL and alveolar bone. Precursor cells have been isolated from human dental follicles of impacted third molars. Recently, human dental follicle progenitor cells showed hard tissue-forming potential in immunocompromised rats (Yagyuu et al. 2010). Dental follicle stem cells may provide a cell source for tissue engineering. Similar to other dental stem cells, these cells form low numbers of adherent clonogenic colonies when released from the tissue following enzymatic digestion (Morsczeck et al. 2005).
The Cell Biology of Amelogenesis
Colin Robinson, Jennifer Kirkham, Roger Shore in Dental Enamel, 2017
With continued morphogenetic movements, a structure is formed with close resemblance to the shape of the future crown (the bell stage). A basal lamina separates the IEE from the dental papilla, and denotes the shape and position of the future dentine-enamel junction. Through a series of epithelial-mesenchymal interactions,5-8 cells of the IEE eventually become cytodifferentiated enamel-matrix secreting ameloblasts (for a more detailed discussion of these interactions, see Chapter 1). Cells of the dental papilla will become the odontoblasts and pulp of the future tooth. Tissue recombination experiments have shown that the dental papilla is responsible for controlling the shape of the developing tooth.9,10 The dental follicle cells (outside the OEE) eventually will take part in the formation of cementum, periodontal ligament, and alveolar bone.11
Benign Oral and Dental Disease
John C Watkinson, Raymond W Clarke, Terry M Jones, Vinidh Paleri, Nicholas White, Tim Woolford in Head & Neck Surgery Plastic Surgery, 2018
The most common developmental odontogenic cyst is the dentigerous cyst (DC) and indeed is the most common cyst of the jaw in the paediatric age group. This cyst arises around the crown of an unerupted tooth with the wall of the cyst attached around the cervical margin of the involved tooth, with significant variation in size. DCs are more commonly unilocular but may also be a multilocular. Radiographically, a dental follicle-related radiolucency around an unerupted tooth should measure less than 4 mm from the surface of the tooth to the outer edge of the follicle; consequently any further increases should raise the possibility of cystic change. It is important to bear in mind that odontogenic tumours such as ameloblastoma and odontogenic keratocyst may involve unerupted teeth and present a diagnostic dilemma.
Relevance of periodic evaluation of endodontically treated primary teeth
Published in Libyan Journal of Medicine, 2019
Sally Kamal El-Din Mohamed, Huda Abutayyem, Said Abdelnabi, Juma Alkhabuli
DC is commonly associated with mandibular 3rd mandibular molar [5]. However, in the current case, the cyst was associated with unerupted mandibular 2nd premolar. Although such cases are relatively uncommon, a few cases have been reported [6]. Shibata et al. [7] studied the occurrence of DC in association with succedaneous teeth during the transitional dentition phase and reported a prevalence of 77.1% in the premolar region. There have been several explanations for the development of inflammatory and non-inflammatory DC. Benn and Altini [8] suggested three pathways for histogenesis of DC. In the first scenario, the developmental DC arises from the dental follicle and becomes secondarily infected as a result of a non-vital tooth. The second form occurs when a permanent successor erupts into radicular cyst that forms at apex of a non-vital deciduous resulting into a DC that is extra follicular in origin. Nevertheless, a radicular cyst developing at apex of primary tooth is extremely rare. The third possible cause is due to spread of peri-apical inflammation from a non-vital deciduous tooth to a follicle of permanent successor.
Dental stem cells for tooth regeneration: how far have we come and where next?
Published in Expert Opinion on Biological Therapy, 2023
At the beginning of tooth development in the first (mandibular) arch of an embryo the tooth germ consists of two tissues: the dental mesoderm, which originates from neural crest cells, and the dental ectoderm, which is part of the surface ectoderm [3,4]. These two types of cells are the origin of the tooth germ and make up the entire tooth [5]. However, during development these two dental cell types become three tissues, one derived from the ectoderm – enamel organ – and two from the mesoderm – dental papilla and dental follicle (dental sac) [6,7]. While the enamel organ is the source of ameloblasts and is heavily involved in tooth crown morphology, some dental epithelial cells become Hertwig’s epithelial root sheath cells involved in tooth root development [8]. The dental mesodermal tissues deliver stem cells for the development of the tooth root and the dental pulp/dentin complex [9]. Interestingly, both dental mesodermal tissues can be harvested from impacted wisdom teeth and their stem cells isolated and used for different applications [10]. In contrast, the enamel organ and most dental ectodermal cells are lost beforehand. Only epithelial rests of Malassez can be obtained for example from impacted wisdom teeth, but a significant number of dental ectodermal progenitor cells cannot be isolated from this source [11,12]. Moreover, these cells are not the genuine progenitors for ameloblasts and it remains nuclear whether they can be used as ectodermal tooth germ cells in whole tooth regeneration approaches, which is the most advanced goal in regenerative dentistry. This article first summarizes the state of the art in tooth engineering.
Role of STRO-1 sorting of porcine dental germ stem cells in dental stem cell-mediated bone tissue engineering
Published in Artificial Cells, Nanomedicine, and Biotechnology, 2018
Gorke Gurel Pekozer, Mustafa Ramazanoglu, Karl Andreas Schlegel, Fatma Nese Kok, Gamze Torun Kose
Stem cells can be isolated from a variety of dental tissues including apical papilla of developing teeth (SCAP) and dental follicle (DFSCs). DFSCs and SCAP are generally cultured by dissecting dental follicle and apical papilla of immature tooth seperately. However, the use of whole tooth germ including dental follicle and its surrounding tissues is an alternative strategy which make use of reciprocal interactions between stem cells of ectoderm and mesoderm origin in order to preserve their stemness [11].
Related Knowledge Centers
- Dental Papilla
- Tooth
- Tooth Development
- Periodontal Fiber
- Periodontium
- Osteoblast
- Cementoblast
- Fibroblast
- Sharpey'S Fibres
- Enamel Organ